New advances in clinical application of neostigmine: no longer focusing solely on increasing skeletal muscle strength

Neostigmine is a clinical cholinesterase inhibitor, that is, commonly used to enhance the function of the cholinergic neuromuscular junction. Recent studies have shown that neostigmine regulates the immune-inflammatory response through the cholinergic anti-inflammatory pathway, affecting perioperative neurocognitive function. This article reviews the relevant research evidence over the past 20 years, intending to provide new perspectives and strategies for the clinical application of neostigmine

Benzyl isothiocyanate induces apoptosis and inhibits tumor growth in canine mammary carcinoma via down-regulation of the cyclin B1/Cdk1 pathway

Background: Canine mammary carcinoma is common in female dogs, and its poor prognosis remains a serious clinical challenge, especially in developing countries. Benzyl isothiocyanate (BITC) has attracted great interest because of its inhibitory effect against tumor activity. However, its effect and the underlying mechanisms of action in canine mammary cancer are not well-understood. Here, we show that BITC suppresses mammary tumor growth, both in vivo and in vitro, and reveal some of the potential mechanisms involved. Methods: The effect of BITC on canine mammary cancer was evaluated on CIPp and CMT-7364, canine mammary carcinoma lines. The cell lines were treated with BITC and then subjected to wound healing and invasion assays. Cell cycles and apoptosis were measured using flow cytometry; TUNEL assay; immunohistochemistry (IHC) for caspase 3, caspase 9, and cyclin D1; hematoxylin and eosin (H&E) staining; and/or quantitative polymerase chain reaction (qPCR). Results: BITC showed a strong suppressive effect in both CIPp and CMT-7364 cells by inhibiting cell growth in vitro; these effects were both dose- and time-dependent. BITC also inhibited migration and invasion of CIPp and CMT-7364 cells. BITC induced G2 arrest and apoptosis, decreasing tumor growth in nude mice by downregulation of cyclin B1 and Cdk1 expression. Conclusion: BITC suppressed both invasion and migration of CIPp and CMT-7364 cells and induced apoptosis. BITC inhibited canine mammary tumor growth by suppressing cyclinB1 and Cdk1 expression in nude mice

Towards joint communication and sensing (Chapter 4)

Localization of user equipment (UE) in mobile communication networks has been supported from the early stages of 3rd generation partnership project (3GPP). With 5th Generation (5G) and its target use cases, localization is increasingly gaining importance. Integrated sensing and localization in 6th Generation (6G) networks promise the introduction of more efficient networks and compelling applications to be developed

Experimental Research into the Repair of High Temperature Damage to Cement Mortar Samples Using Microbial Mineralization Technology

Experiments such as microbial activation culture, subculture selection, and fire damage repair of cement mortar specimens were conducted to investigate the repairing effect of Sporosarcina pasteurii as a repair agent for fire-damaged cracks in cement mortar specimens. In addition, multi-scale parameters such as compressive strength and chloride ion migration coefficient of cement mortar specimens before and after restoration were compared. The effect of microbial mineralization technology on the repair of fire-damaged cracks in cement mortar specimens was investigated, and the microstructure and mineral composition of the products were analyzed. The results showed that the strong alkaline environment in the cracks of the cement mortar specimens after a high temperature of 500 °C inhibited the activity of bacteria and weakened the mineralization effect; the compressive strength of the repaired cement mortar specimens was 22.8% higher than that of the unrepaired fire-damaged specimens; the compressive strength of the repaired cement mortar reached 78.2% of the strength of the original cement mortar specimen without high temperature; after restoration, the chloride ion penetration resistance of the cement mortar specimens decreased by about 16.9% compared with that before restoration

A High-Precision Positioning Scheme Under Non-Point Visible Transmitters

International audienceAn interacting multiple model based on unscented Kalman filter (IMM-UKF) is widely applied to positioning and tracking targets in various tracking scenarios. At the same time, visible light positioning (VLP) is developing rapidly due to the low cost and accuracy. Therefore, indoor positioning and tracking based on VLP combined with the IMM-UKF has attracted considerable interest. However, existing algorithms work on the assumption that the light-emitting diodes used for tracking are all point light sources, which ignores the geometry of these transmitters and results in low tracking accuracy. To overcome this problem, this paper proposes an innovative tracking algorithm based on VLP. This algorithm considers the shapes of the lights in combination with existing tracking algorithms, such as IMM-UKF. Simulation results show that in a standard Gaussian noise environment, the larger the transmitter is, the more meaningful the proposed algorithm is. INDEX TERMS Target positioning and tracking, visible light positioning (VLP), interacting multiple model (IMM), unscented Kalman filter (UKF), shapes of transmitters

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

Background/Aims: Lung adenocarcinoma, a form of non-small cell lung cancer with high lethality at an advanced stage, is becoming more common in women, non- or never-smokers, and even young adults. At present, there are still no effective early diagnosis methods for patients to be cured in a timely manner. Circular RNAs (circRNAs), which are stable and conserved non-coding RNA in mammalian cells, have been reported to be widely involved in the processes of cancer disease. However, it is still a puzzle as to which specific circRNAs are involved in the development of early-stage lung adenocarcinoma. Methods: Tumor samples and paired adjacent normal tissues from 4 patients with early-stage lung adenocarcinoma were selected to investigate the expression profile of circRNAs by using a high-throughput circRNA microarray. Bioinformatic analyses were conducted to screen those differentially expressed circRNAs. qRT-PCR and sequencing were performed to assure the microarray data. Results: A total of 357 circRNAs were dysregulated in the tumor samples, which suggests potential roles in lung cancer. qRT-PCR detection showed that five selected circRNAs were identical to the microarray data, and the potential circRNA-miRNA interactions were predicted. Conclusion: This work illustrates that clusters of circRNAs are aberrantly expressed in early-stage lung adenocarcinoma, which might offer potential targets for the early diagnosis of this disease and new genetic insights into lung cancer

Rapid Method for Identification of Six Common Species of Mycobacteria Based on Multiplex SNP Analysis▿

A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobacterial species by the multiplex SNaPshot method. Of the 468 mycobacterial isolates, 464 (99.15%) could be correctly identified by this assay. The multiplex SNaPshot technique is a promising discriminatory tool for rapid and accurate identification of frequently encountered clinical mycobacterial species

Initial experience of thoracoscopic segmentectomy of basal segment through the inferior pulmonary ligament approach in treating congenital lung malformations in children

Abstract Purpose This study aimed to evaluate the feasibility and limitations of thoracoscopic segmentectomy of the basal segment (S10). Methods Clinical data of 15 children with congenital lung malformations (CLM) who underwent thoracoscopic segmentectomy of S10 via the inferior pulmonary ligament approach from January to October 2022 were retrospectively analyzed. The demographics, clinical presentation, intraoperative time, blood loss, postoperative events, and follow-up duration were assessed. Results There were 15 patients in this group (nine males and six females). Age ranges from 4.3 to 96.0 months (median, 7.7 months). Fourteen patients underwent S10 segmentectomy, with one undergoing right S10 segmentectomy and right S6 partial wedge resection. The surgical time was 57–125 min (median, 80 min), intraoperative bleeding volume (5–20 ml; median, 10 ml), postoperative drainage tube indwelling (2–4 d; median, 3 d), and postoperative hospitalization time (4–7 d; median, 5 d). No intraoperative conversions, surgical mortalities, or major complications were observed among these patients. Subcutaneous emphysema appeared in three patients; however, it disappeared following conservative observation without pneumothorax or bronchopleural fistula occurrence. Conclusions Thoracoscopic segmentectomy of S10 via the inferior pulmonary ligament approach is technically feasible for treating CLM; however, this surgical approach may have certain limitations for CLM with large cysts